Fig. 2. Neurocan cleavage by ADAMTSs. A. In vitro digestion of murine neurocan by ADAMTSs. Neurocan was incubated with ADAMTS1 (NCAN + ADAMTS1), ADAMTS4 (NCAN + ADAMTS4), ADAMTS5 (NCAN + ADAMTS5), or ADAMTS12 (NCAN + ADAMTS12) for 16 h. NCAN indicates neurocan incubated alone (without metalloproteases, control). Intervening irrelevant lanes are not shown. The presence of the proteolytic products of ADAMTS12 activity is indicated with arrows. Molecular weight markers are indicated on the left (kDa) B. Neurocan was incubated in the presence (+) or absence (−) of ADAMTS12 for 0, 4, 8, and 12 h. Molecular weight markers are indicated on the left (kDa). C. Analysis of the expression of ADAMTS12 (top) and neurocan (NCAN, bottom) in control and RNAi (shADAMTS12) SIM-A9 cells. Samples were loaded in triplicate. Molecular weight markers are indicated on the left (kDa). β-Actin was employed as a loading control.